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Amperometric determination of formaldehyde via the hexacyanoferrate(III) -coupled dehydrogenase reaction by Jui-Lin Weng; Mat H. Ho; William K. Nonidez is a Chemistry article available to read on EtoBox.

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An enzymatic method with amperometrlc detectlon was developed for the determmatlon of formaldehyde Formaldehyde 1s first oxldlzed by reactlon with NAD+ m the presence of formaldehyde dehydrogenase. The resultmg NADH 1s then oxldlzed by hexacyanoferrate(II1) m the presence of dlaphorase to produce hexacyanoferrate(I1) The anodlc current generated by oxldatlon of the hexacyanoferrate(I1) at the surface of a glassy carbon working electrode, held at a potential of 0 40 V vs. an Ag/AgCI reference electrode, 1s measured. The effects of solution condltlons are exammed and a hnear relatlonshlp between rate of current change and formaldehyde concentration 1s obtamed from 0 01 to 0 5 gg ml-' with a correlation coefflclent of 0.9998 The relative standard devlatlon for the proposed method 1s 6 4% at 0.01 gg ml-' formaldehyde and 0 88% at 0.5 gg ml-'

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Author

Jui-Lin Weng; Mat H. Ho; William K. Nonidez

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0003-2670)

Published

1990

Language

EN

Field

Chemistry (Physical Sciences)