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[15] Improved vectors for plant transformation: Expression cassette vectors and new selectable markers by S.G. Rogers; H.J. Klee; R.B. Horsch; R.T. Fraley is a book available to read on EtoBox.

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IMPROVED VECTORS FOR PLANT TRANSFORMATION 253 somes ranging in size from 270 to 29 kb, but we could also compare the stabilities of telocentric, acrocentric, and metacentric chromosomes of similar size. The technique described here may be used to construct other types of rearrangements. Chromosomal translocations may be generated by transforming cells with a DNA fragment that contains sequences from one chromosome at one end of the fragment and sequences from another chromosome at the other end. These translocations must be made such that acentric or dicentric chromosomes are not generated. It also may be possible to fuse two chromosomes together, provided that the centromere of one of the chromosomes to be fused can be deleted simultaneously.By changing the orientation of the homologous sequences on the DNA fragment, we constructed a ring derivative of chromosome III. Studies on artificial circular chromosomes have suggested that factors affecting their mitotic stability may be different from those affecting linear chromosomes. 2° Using this method, the mitotic behavior of various circular derivatives of a natural chromosome could be investigated. The ability to readily circulariz

Author

S.G. Rogers; H.J. Klee; R.B. Horsch; R.T. Fraley

Published

1987

Language

EN