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Can I read Kinetics of wound-induced v-Ha-ras transgene expression and papilloma development in transgenic Tg.AC mice on EtoBox?
Kinetics of wound-induced v-Ha-ras transgene expression and papilloma development in transgenic Tg.AC mice by Ronald E. Cannon; Judson W. Spalding; Carol S. Trempus; Carl J. Szczesniak; Kelly M. Virgil; Michael C. Humble; Raymond W. Tennant is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Kinetics of wound-induced v-Ha-ras transgene expression and papilloma development in transgenic Tg.AC mice about?
The Tg.AC transgenic mouse, which harbors an activated v-Ha-ras coding region that is fused to an embryonic zeta globin transcriptional control region and a 3' simian virus 40 polyadenylation sequence, rapidly develops epidermal papillomas in response to topical application of chemical carcinogens or tumor promoters or to full-thickness wounding of the dorsal skin. In this report, we investigated the localization and temporal induction of v-Ha-ras transgene expression after full-thickness wounding of Tg.AC mouse skin. Surgically inflicted full-thickness incisions 3 cm long yielded four to six papillomas per Tg.AC mouse by 5 wk after wounding. Similar wounding of the FVB/N isogenic host strain did not produce tumors, which implicates a causal role for the v-Ha-ras transgene. Reverse transcription-polymerase chain reaction assays detected the v-Ha-ras transgene transcript in total RNA samples isolated from wound-associated tissue 3 and 4 wk after wounding. Tissues 1-2 wk after wounding and all non-wound-associated tissues were negative for transgene expression. In situ hybridization experiments using transgene-specific 35S-labeled antisense RNA probes localized transgene expression t
Who reads Kinetics of wound-induced v-Ha-ras transgene expression and papilloma development in transgenic Tg.AC mice?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Ronald E. Cannon; Judson W. Spalding; Carol S. Trempus; Carl J. Szczesniak; Kelly M. Virgil; Michael C. Humble; Raymond W. Tennant
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0899-1987)
- Published
- 1997
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)