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Identification and Characterisation of the Lactic Acid Bacteria Associated with the Traditional Fermentation of Dairy Fermented Product by Promiselynda Ijeoma Obioha; L. Irene I. Ouoba; Amarachukwu Anyogu; Brigitte Awamaria; Sarah Atchia; Philippa C. Ojimelukwe; Jane P. Sutherland; Hamid B. Ghoddusi is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Identification and Characterisation of the Lactic Acid Bacteria Associated with the Traditional Fermentation of Dairy Fermented Product about?
The aim of this research was to identify the key lactic acid bacteria associated with the fermentation of dairy traditional fermented products for developing starter cultures for controlled fermentation. A total of 100 lactic acid bacteria (LAB) were isolated from dairy traditional fermented products. Samples were obtained from eight producers in the South East of Nigeria. Isolates were identified by phenotypic and genotypic techniques including rep-PCR genotyping and sequencing of the 16S rRNA, pheS and rpoA genes. Isolates were characterised for antimicrobial activity against foodborne pathogens, exopolysaccharide (EPS) production and survival at low pH and in the presence of bile salts. All isolates clustered into 11 distinct rep-PCR groups and were identified as Lactobacillus fermentum (40%), Lactobacillus delbrueckii (23%), Streptococcus thermophilus (22%), Streptococcus infantarius (10%), Lactobacillus senioris (2%), Leuconostoc pseudomesenteriodes (2%) and Enterococcus thailandicus (1%). Lactobacillus fermentum showed a broad spectrum antimicrobial activity and survival at low pH, while Lactobacillus delbrueckii was able to tolerate low pH and produce EPS. All isolates survi
Who reads Identification and Characterisation of the Lactic Acid Bacteria Associated with the Traditional Fermentation of Dairy Fermented Product?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Promiselynda Ijeoma Obioha; L. Irene I. Ouoba; Amarachukwu Anyogu; Brigitte Awamaria; Sarah Atchia; Philippa C. Ojimelukwe; Jane P. Sutherland; Hamid B. Ghoddusi
- Publisher
- Springer Science and Business Media LLC
- Published
- 2021
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)