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Can I read Cibacron Blue-Eupergit, an affinity matrix for soybean (Glycine max) phospholipase A2 purification on EtoBox?
Cibacron Blue-Eupergit, an affinity matrix for soybean (Glycine max) phospholipase A2 purification by R. Madoery; M. Minchiotti is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Cibacron Blue-Eupergit, an affinity matrix for soybean (Glycine max) phospholipase A2 purification about?
Cibacron Blue, a monochlorotriazine anthraquinone dye was covalently coupled to Eupergit C, an acrylic polymer support containing oxirane groups. The developed affinity adsorbent matrix was effective in the purification of phospholipase A 2 from soybean (Glycine max) seeds. The coupling chemistry applied in the obtention of the affinity system was simple, not altering the interaction between phospholipase A 2 and the immobilized ligand. The system has the advantage that both, the ligand and the support are chemical and microbiologically stable. Performing a classical column chromatography approach, phospholipase A 2 was purified about 23 folds (purification factor). In the experimental conditions, the enzyme recovery (activity units) was 13.4%. The purified enzyme obtained by this approach could be applied in biotechnological processes. Lysophospholipids, derivatives obtained in the hydrolysis reaction promoted by phospholipase A 2 , are potent biosurfactants useful in food industry and for medical purposes.
Who reads Cibacron Blue-Eupergit, an affinity matrix for soybean (Glycine max) phospholipase A2 purification?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- R. Madoery; M. Minchiotti
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0141-0229)
- Published
- 2006
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)