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Influence of Changing Pulse Repetition Frequency on Chemical and Biological Effects Induced by Low-intensity Ultrasound in Vitro by Mikhail A. Buldakov; Mariame A. Hassan; Qing-Li Zhao; Loreto B. Feril Jr.; Nobuki Kudo; Takashi Kondo; Nikolai V. Litvyakov; Mikhail A. Bolshakov; Vladislav V. Rostov; Nadejda V. Cherdyntseva; Peter Riesz is a Engineering article available to read on EtoBox.
What is Influence of Changing Pulse Repetition Frequency on Chemical and Biological Effects Induced by Low-intensity Ultrasound in Vitro about?
This study was undertaken to examine ultrasound (US) mechanisms and their impact on chemical and biological effects in vitro as a function of changing pulse repetition frequency (PRF) from 0.5 to 100 Hz using a 1 MHz-generator at low-intensities and 50% duty factor (DF). The presence of inertial cavitation was detected by electron paramagnetic resonance (EPR) spin-trapping of hydroxyl radicals resulting from sonolysis of water. Non-cavitational effects were evaluated by studying the extent of sucrose hydrolysis measured by UV spectrophotometry. Biological effects were assessed by measuring the extent of cell killing and apoptosis induction in U937 cells using Trypan blue dye exclusion test and flow cytometry, respectively. The results indicate significant PRF dependence with respect to hydroxyl radical formation, cell killing and apoptosis induction. The lowest free radical formation and cell killing and the highest cell viability were found at 5 Hz (100 ms pulse duration). On the other hand, no correlation was found between sucrose hydrolysis and PRF. To our knowledge, this is the first report to be devoted to study the impact of low PRFs at low-intensities on US-induced chemical
Who reads Influence of Changing Pulse Repetition Frequency on Chemical and Biological Effects Induced by Low-intensity Ultrasound in Vitro?
It is typically read by researchers, students, and practitioners in Engineering.
- Author
- Mikhail A. Buldakov; Mariame A. Hassan; Qing-Li Zhao; Loreto B. Feril Jr.; Nobuki Kudo; Takashi Kondo; Nikolai V. Litvyakov; Mikhail A. Bolshakov; Vladislav V. Rostov; Nadejda V. Cherdyntseva; Peter Riesz
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 1350-4177)
- Published
- 2009
- Language
- EN
- Field
- Engineering (Physical Sciences)