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Can I read Low Molecular Weight pDMAEMA-block-pHEMA Block-Copolymers Synthesized via RAFT-Polymerization: Potential Non-Viral Gene Delivery Agents? on EtoBox?
Low Molecular Weight pDMAEMA-block-pHEMA Block-Copolymers Synthesized via RAFT-Polymerization: Potential Non-Viral Gene Delivery Agents? by Olga Samsonova; Christian Pfeiffer; Markus Hellmund; Olivia M. Merkel; Thomas Kissel is a Materials Science article available to read on EtoBox.
What is Low Molecular Weight pDMAEMA-block-pHEMA Block-Copolymers Synthesized via RAFT-Polymerization: Potential Non-Viral Gene Delivery Agents? about?
The aim of this study was to investigate non-viral pDNA carriers based on diblock-copolymers consisting of poly(2-(dimethyl amino)ethyl methacrylate) (pDMAEMA) and poly(2-hydroxyethyl methacrylate) (pHEMA). Specifically the block-lengths and molecular weights were varied to determine the minimal requirements for transfection. Such vectors should allow better transfection at acceptable toxicity levels and the entire diblock-copolymer should be suitable for renal clearance. For this purpose, a library of linear poly(2-(dimethyl amino)ethyl methacrylate-block-poly(2-hydroxyl methacrylate) (pDMAEMA-block-pHEMA) copolymers was synthesized via RAFT (reversible additionfragmentation chain transfer) polymerization in a molecular weight (Mw) range of 17-35.7 kDa and analyzed using 1 H and 13 C NMR (nuclear magnetic resonance), ATR (attenuated total reflectance), GPC (gel permeation chromatography) and DSC (differential scanning calorimetry). Copolymers possessing short pDMAEMA-polycation chains were 1.4-9.7 times less toxic in vitro than polyethylenimine (PEI) 25 kDa, and complexed DNA into polyplexes of 100-170 nm, favorable for cellular uptake. The DNA-binding affinity and polyplex stabil
Who reads Low Molecular Weight pDMAEMA-block-pHEMA Block-Copolymers Synthesized via RAFT-Polymerization: Potential Non-Viral Gene Delivery Agents??
It is typically read by researchers, students, and practitioners in Materials Science.
- Author
- Olga Samsonova; Christian Pfeiffer; Markus Hellmund; Olivia M. Merkel; Thomas Kissel
- Publisher
- MDPI AG
- Published
- 2011
- Language
- EN
- Field
- Materials Science (Physical Sciences)