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Silica-based Monolithic Columns with Mixed-mode Reversed-phase/weak Anion-exchange Selectivity Principle for High-performance Liquid Chromatography by Raquel Nogueira; Dieter Lubda; Alexander Leitner; Wolfgang Bicker; Norbert M. Maier; Michael Lämmerhofer; Wolfgang Lindner is a Chemistry article available to read on EtoBox.
What is Silica-based Monolithic Columns with Mixed-mode Reversed-phase/weak Anion-exchange Selectivity Principle for High-performance Liquid Chromatography about?
This article describes the synthesis, chromatographic characterization, and performance evaluation of analytical (10064.6 mm id) and semipreparative (100610 mm id) monolithic silica columns with mixed-mode RP/weak anion-exchange (RP/WAX) surface modification. The monolithic RP/WAX columns were obtained by immobilization of N-(10-undecenoyl)-3-aminoquinuclidine onto thiol-modified monolithic silica columns (Chromolithm) by a radical addition reaction. Their chromatographic characterization by Engelhardt and Tanaka tests revealed slightly lower hydrophobic selectivities than C-8 phases, as well as higher polarity and also improved shape selectivity than RP-18e silica rods. The surface modification enabled separation by both RP and anion-exchange chromatography principles, and thus showed complementary selectivities to the RP-18e monoliths. The mixed-mode monoliths have been tested for the separation of peptides and turned out to be particularly useful for hydrophilic acidic peptides, which are usually insufficiently retained on RP-18e monolithic columns. Compared to a corresponding particulate RP/WAX column (5 lm, 10 nm pore diameter), the analytical RP/WAX monolith caused lower syst
Who reads Silica-based Monolithic Columns with Mixed-mode Reversed-phase/weak Anion-exchange Selectivity Principle for High-performance Liquid Chromatography?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Raquel Nogueira; Dieter Lubda; Alexander Leitner; Wolfgang Bicker; Norbert M. Maier; Michael Lämmerhofer; Wolfgang Lindner
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley (ISSN 1615-9306)
- Published
- 2006
- Language
- EN
- Field
- Chemistry (Physical Sciences)