Can I read Fluoro-Ruby as a reliable marker for regenerating fiber tracts on EtoBox?

Fluoro-Ruby as a reliable marker for regenerating fiber tracts by Christine Radtke; Jeffery D. Kocsis; Wolfgang Baumgärtner; Peter M. Vogt is a Medicine article available to read on EtoBox.

What is Fluoro-Ruby as a reliable marker for regenerating fiber tracts about?

## Abstract Axon visualization techniques are important in assessing the efficacy of interventional approaches to stimulate neural regeneration. Whereas the labeling of descending tracts in the spinal cord has been well established using the intracortical injection of biotin dextran amine (BDA), the labeling of ascending sensory fibers of the dorsal funiculus is more problematic. Fluoro-Ruby (FR; dextran tetramethylrhodamine; MW 10,000) is a bidirectional permanent tracer, but the retrograde tracing of fibers is particularly prominent, and FR is a highly sensitive tracer that can be applied in discrete injection sites. In the present report, we used FR to efficiently label ascending fibers in the dorsal columns of the rat spinal cord. After transplantation of olfactory ensheathing cells into the transected dorsal funiculus, the application of FR was able to detect regenerating ascending fibers in the spinal cord. Regenerated fibers crossing the injury site were labeled and easily identified. It is likely that the tracer was taken up by damaged fibers. As additional advantages, the labeling is resistant to photobleaching and no additional tissue processing is necessary for visualiza

Who reads Fluoro-Ruby as a reliable marker for regenerating fiber tracts?

Author

Christine Radtke; Jeffery D. Kocsis; Wolfgang Baumgärtner; Peter M. Vogt

Publisher

Walter de Gruyter GmbH

Published

2017

Language

EN

Field

Medicine (Health Sciences)