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Can I read Greenhouse test of spraying dsRNA to control the western flower thrips, Frankliniella occidentalis , infesting hot peppers on EtoBox?
Greenhouse test of spraying dsRNA to control the western flower thrips, Frankliniella occidentalis , infesting hot peppers by Falguni Khan; Minlee Kim; Yonggyun Kim is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Greenhouse test of spraying dsRNA to control the western flower thrips, Frankliniella occidentalis , infesting hot peppers about?
## Background The western flower thrips __Frankliniella occidentalis__ is an insect pest that damages various crops, including hot peppers. It is a vector of a plant pathogen, tomato spotted wilt virus. To control this pest, chemical insecticides have been used in the past, but the control efficacy is unsatisfactory owing to rapid resistance development by __F. occidentalis__. ## Methodology : This study reports a novel control technology against this insect pest using RNA interference (RNAi) of the vacuolar-type ATPase (__vATPase__) expression. Eight subunit genes (__vATPase-A__ ∼ __vATPase-H__) of vATPase were obtained from the __F. occidentalis__ genome and confirmed for their expressions at all developmental stages. ## Results Double-stranded RNAs (dsRNAs) specific to the eight subunit genes were fed to larvae and adults, which significantly suppressed the corresponding gene expressions after 24-h feeding treatment. These RNAi treatments resulted in significant mortalities, in which the dsRNA treatments at ∼2,000 ppm specific to __vATPase-A__ or __vATPase-B__ allowed complete control efficacy near 100% mortality in 7 days after treatment. To prevent dsRNA degradation by the dig
Who reads Greenhouse test of spraying dsRNA to control the western flower thrips, Frankliniella occidentalis , infesting hot peppers?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Falguni Khan; Minlee Kim; Yonggyun Kim
- Publisher
- Springer Science and Business Media LLC
- Published
- 2023
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)