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Crosstalk Between Adipose-derived Stem/stromal Cells and Vocal Fold Fibroblasts in Vitro by Yoshihiko Kumai; James B. Kobler; Hyoungshin Park; Gerardo Lopez-Guerra; Sandeep Karajanagi; Victoria L.M. Herrera; Steven M. Zeitels is a Medicine article available to read on EtoBox.
What is Crosstalk Between Adipose-derived Stem/stromal Cells and Vocal Fold Fibroblasts in Vitro about?
## Abstract ## Objectives/Hypothesis: To explore adipose‐derived stem cell/fibroblast interactions as a potential remodeling pathway for vocal fold scar. ## Study Design: Fibroblasts and adipose‐derived stem/stromal cells (ASCs) were cultured alone and in combination in a cell‐contact–independent paracrine system. Analyses of cell proliferation, and the production of hyaluronic acid (HA) and collagen were performed on samples collected on days 1, 3, and 7. ## Methods: Normal fibroblasts (NFs) were isolated bilaterally from the subepithelial lamina propria of two normal ferret vocal folds. Scar fibroblasts (SFs) were isolated from vocal folds that were electrocauterized 2 weeks before harvest. ASCs were isolated from lipoaspirated subcutaneous abdominal fat of two ferrets. A transwell cell‐contact–independent cell communication culturing system was used for coculture experiments. Cells were seeded at 50,000/well in both monoculture and coculture experiments. ## Results: In monoculture, SFs proliferated faster and produced less HA and more collagen than NFs at day 7 (__P__ < .05). In SF/ASC coculture, SF proliferation was diminished and collagen production at day 7 decreased (__P__ <
Who reads Crosstalk Between Adipose-derived Stem/stromal Cells and Vocal Fold Fibroblasts in Vitro?
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- Author
- Yoshihiko Kumai; James B. Kobler; Hyoungshin Park; Gerardo Lopez-Guerra; Sandeep Karajanagi; Victoria L.M. Herrera; Steven M. Zeitels
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); Wiley-Blackwell; Wiley (ISSN 0023-852X)
- Published
- 2009
- Language
- EN
- Field
- Medicine (Health Sciences)