Can I read Bioanalysis and pharmacokinetics of chitosan ester in rabbit serum by HPLC with postcolumn fluorescence derivatization on EtoBox?
Bioanalysis and pharmacokinetics of chitosan ester in rabbit serum by HPLC with postcolumn fluorescence derivatization by Yuhong Han; Zhihua Lv; Tingfu Jiang; Yuanhong Wang is a Chemistry article available to read on EtoBox.
What is Bioanalysis and pharmacokinetics of chitosan ester in rabbit serum by HPLC with postcolumn fluorescence derivatization about?
Interest in antiatherosclerotic activity of chitosan ester (PS916) with a new form of sulfate amino polysaccharide derived from marine chitin has necessitated the development of a sensitive and specific method to study its pharmacokinetics. A sensitive and reproducible high-performance liquid chromatography (HPLC) with postcolumn fluorescence derivatization method was developed and validated for the determination of PS916 in rabbit serum. Chromatography was carried out using a C8 reversed-phase column with an isocratic mobile phase consisting of methanol-water (20:80, v/v) at a flow rate of 0.2 ml/min. The derivatization procedure involved postcolumn reaction with guanidine hydrochloride in an alkaline medium at 110 • C. The fluorometric detector was operated at 250 nm (excitation) and 435 nm (emission). The assay was linear over the concentration range of 5-100 g/ml. The lower limit of detection (LLOD) was found to be 1.0 g/ml. The proposed method was successfully applied for a pharmacokinetic study of PS916 in rabbits.
Who reads Bioanalysis and pharmacokinetics of chitosan ester in rabbit serum by HPLC with postcolumn fluorescence derivatization?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Yuhong Han; Zhihua Lv; Tingfu Jiang; Yuanhong Wang
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-376X)
- Published
- 2007
- Language
- EN
- Field
- Chemistry (Physical Sciences)