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Characterization of a bovine pregnancy-associated protein using two-dimensional gel electrophoresis, N-terminal sequencing and mass spectrometry by Jaehoon Pyo; Sun-Il Hwang; Jisun Oh; So-Jin Lee; Sun-Cheol Kang; Jong-Sang Kim; Jinkyu Lim is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
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## Abstract Bovine pregnancy‐associated protein (bPAP) isolated from pregnant bovine urines by two‐dimensional electrophoresis (2‐DE) was characterized by __N__‐terminal sequencing, internal sequencing, and mass spectrometric analyses using matrix‐assisted laser desorption/ionization‐time of flight mass spectrometry and tandem mass spectrometry. Database search using the amino acid sequences and the peptide mass profiles showed that the protein is a novel bovine pregnancy‐associated protein of which the __N__‐terminus has a high similarity to collagen alpha. The protein has a molecular mass of 21 kDa and a p__I__ of 6.1. The expression profiles of the protein from the urine of 30 pregnant and 20 nonpregnant cows characterized by 2‐DE indicated that the expression of bPAP during pregnancy increased to over nmol from the pmol level basal expression of bPAP at the nonpregnant state with < 3% of false negatives and < 10% of false positives. Using the peptide sequence information, polyclonal antibodies against the bPAP protein were generated. The purified polyclonal antibodies against the peptide sequences of bPAP detected the 21 kDa protein on the blots of pregnant cow urine by Western
Who reads Characterization of a bovine pregnancy-associated protein using two-dimensional gel electrophoresis, N-terminal sequencing and mass spectrometry?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Jaehoon Pyo; Sun-Il Hwang; Jisun Oh; So-Jin Lee; Sun-Cheol Kang; Jong-Sang Kim; Jinkyu Lim
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley; Research Square (ISSN 1615-9853)
- Published
- 2003
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)