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Isolation and identification of two isomeric forms of malonyl-coenzyme A in commercial malonyl-coenzyme A by Paul E Minkler; Vernon E Anderson; Nakul C Maiti; Janos Kerner; Charles L Hoppel is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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Two isomers of malonyl-coenzyme A (malonyl-CoA) were detected in a commercial preparation of malonyl-CoA. These compounds were separated by preparative high-performance liquid chromatography (HPLC) and characterized by HPLC/ultraviolet (UV)/mass spectrometry. Both compounds had a UV absorbance maximum at 259-260 nm. Both compounds underwent negative electrospray ionization to produce a [M-H] ¡ quasi-molecular ion at m/z 852 and both compounds underwent collision-induced dissociation to produce a characteristic fragment at m/z 808, all consistent with the structure of malonyl-CoA. Nuclear magnetic resonance spectrometry showed that the two chromatographically distinguishable malonyl-CoAs are structural isomers: the major component is the naturally occurring malonyl-CoA and the contaminant is 3 0 -dephospho-2 0 -phospho-coenzyme A.

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Author

Paul E Minkler; Vernon E Anderson; Nakul C Maiti; Janos Kerner; Charles L Hoppel

Publisher

Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 0003-2697)

Published

2004

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)