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DNA Methylation of Alternative Promoters Directs Tissue Specific Expression of Epac2 Isoforms by Erling A. Hoivik; Solveig L. Witsoe; Inger R. Bergheim; Yunjian Xu; Ida Jakobsson; Anders Tengholm; Stein Ove Doskeland; Marit Bakke is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is DNA Methylation of Alternative Promoters Directs Tissue Specific Expression of Epac2 Isoforms about?

Epac 1 and Epac 2 (Epac1/2; exchange factors directly activated by cAMP) are multidomain proteins that mediate cellular responses upon activation by the signaling molecule cAMP. Epac1 is ubiquitously expressed, whereas Epac2 exhibits a restricted expression pattern. The gene encoding Epac2 gives rise to at least three protein isoforms (Epac2A, Epac2B and Epac2C) that exhibit confined tissue and cell specific expression profiles. Here, we describe alternative promoter usage for the different isoforms of Epac2, and demonstrate that the activity of these promoters depend on the DNA methylation status. Bisulfite sequencing demonstrated that the level of methylation of the promoters in different tissues correlates with Epac2 isoform expression. The presented data indicate that the tissue-specific expression of the Epac2 isoforms is epigenetically regulated, and identify tissue-specific differentially methylated promoter regions within the __Epac2__ locus that are essential for its transcriptional control.

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Author

Erling A. Hoivik; Solveig L. Witsoe; Inger R. Bergheim; Yunjian Xu; Ida Jakobsson; Anders Tengholm; Stein Ove Doskeland; Marit Bakke

Publisher

Public Library of Science (PLoS)

Published

2013

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)