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Long term anoxia in rainbow trout investigated by 2‐DE and MS/MS by Tune Wulff; Flemming Jessen; Peter Roepstorff; Else K. Hoffmann is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract Twenty‐four hours of N~2~ induced anoxia induced global perturbations on protein expression in rainbow trout hypodermal fibroblasts cell line. Anoxia was obtained by depleting the medium of O~2~ by flushing with N~2~, and protein changes were studied by 2‐DE coupled with MS providing quantitative measurements of a large number of proteins in one single study. The anoxic insult changed the level of 33 protein spots: 22 of these were up‐regulated compared to the control situation and 11 were down‐regulated. Using MS/MS sequencing 19 of the 33 protein spots that changed were identified, corresponding to a success rate of more than 50%. The identified proteins included two proteins involved in energy metabolism namely phosphoglycerate mutase and isocitrate dehydrogenase. In addition we observed the up‐regulation of a cluster of proteins that contribute to cytoskeleton function. These are calpain, EB1, and Rho GDP dissociation inhibitor (GDI). The up‐regulation of Rho GDI was shown to develop in a time dependent manner with no significant increase for up to 8 h of anoxia. In conclusion, this study provides a thorough investigation of the effect of anoxia in a cell line from

Who reads Long term anoxia in rainbow trout investigated by 2‐DE and MS/MS?

Author

Tune Wulff; Flemming Jessen; Peter Roepstorff; Else K. Hoffmann

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley; Research Square (ISSN 1615-9853)

Published

2008

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)