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Ceramide Quantification via LC-ESI-MS/MS by Siti Fatriyah is a document available to read on EtoBox.
This document details a validated liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) method for quantifying ceramide species in biological samples, achieving detection limits of 5–50 pg/ml. The method demonstrated high recovery rates from human plasma and rat tissues, and was successfully applied to show that exercise training reduces plasma ceramide levels in obese adults. The technique is versatile and can be adapted for other sphingolipids with minimal modifications.
- Author
- Siti Fatriyah
- Language
- EN