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Development of an Electrochemical Method for the Rapid Determination of Microbial Concentration and Evidence for the Reaction Mechanism by G. Ramsay; A.P.F. Turner is a Chemistry article available to read on EtoBox.

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A rapid amperometric method for the determination of microbial concentration is described. The micro-organisms reduce the redox mediator, potassium hexacyanoferrate (III), which is reoxidized to yield a current proportional to the microbial concentration. Escherichia coli gave a linear response (standard error (S,) ~0.17, r=0.9999, n=5, P>99.9%) over the concentration range 106-10' cells ml-' with a response time of less than 1 min. The effect of varying temperature showed that the electrochemical response of the bacteria was linked to respiration rate. Studies with respiratory inhibitors suggested that hexacyanoferrate (III) is reduced by the segment of the respiratory chain between nicotinamide adenine dinucleotide (NADH) dehydrogenase and the terminal oxidases.

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Author

G. Ramsay; A.P.F. Turner

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0003-2670)

Published

1988

Language

EN

Field

Chemistry (Physical Sciences)