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In vitro selection of DNA aptamers and their integration in a competitive voltammetric biosensor for azlocillin determination in waste water by Raja Chinnappan; Shimaa Eissa; Atheer Alotaibi; Ayesha Siddiqua; Omar A. Alsager; Mohammed Zourob is a Chemistry article available to read on EtoBox.
What is In vitro selection of DNA aptamers and their integration in a competitive voltammetric biosensor for azlocillin determination in waste water about?
The uncontrolled usage of veterinary antibiotics has led to their widespread pollution in waterways and milk products. Potential impact of antibiotic residues on the environment and human health such as increased antibiotic resistance of microorganisms and triggering allergic reactions in humans have been reported. In this work, we developed a highly selective and sensitive voltammetric aptasensor for on-step, sensitive and low cost detection of azlocillin antibiotic, one of the broad spectrum β-lactam antibiotics. The successful selection of DNA aptamers against azlocillin was accomplished using systemic evolution of ligands by exponential enrichment (SELEX) method. Fluorescence-binding assays showed dissociation constant of 55 nM for one of the selected aptamers (Az9). This aptamer was used to construct a competitive voltammetric aptasensor for azlocillin. A limit of detection of 1.2 pg/mL as well as remarkable selectivity against potential interfering agents, including amoxicillin, were achieved. This signal-off competitive sensor takes 30-50 min to complete the quantification of the target antibiotic. The sensor was challenged by detecting the target directly in complex environ
Who reads In vitro selection of DNA aptamers and their integration in a competitive voltammetric biosensor for azlocillin determination in waste water?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Raja Chinnappan; Shimaa Eissa; Atheer Alotaibi; Ayesha Siddiqua; Omar A. Alsager; Mohammed Zourob
- Published
- 2020
- Language
- EN
- Field
- Chemistry (Physical Sciences)