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Galectin-3 Promotes Neural Cell Adhesion and Neurite Growth by Penka Pesheva; Stephan Kuklinski; Brigitte Schmitz; Rainer Probstmeier is a Neuroscience article available to read on EtoBox.

What is Galectin-3 Promotes Neural Cell Adhesion and Neurite Growth about?

Galectin-3 is a member of the galectin family and belongs to a group of soluble ␤-galactoside-binding animal lectins. The molecule is expressed by neural and nonneural cells intra-(cytoplasm and nucleus) as well as extra-cellularly (plasma membrane and extracellular space). By using an in vitro cell-substratum adhesion assay, we have addressed the question whether galectin-3 present in the extracellular milieu may support the adhesion and/or neurite outgrowth of neural cells in a manner analogous to cell adhesion molecules. Galectin-3 was immobilized as a substratum and various cell types, N2A (neuroblastoma), PC12 (pheochromocytoma), and TSC (transformed Schwann cells) cell lines, neural cells from early postnatal mouse cerebellum, and dorsal root ganglion neurons from newborn mice were allowed to adhere to the lectin. Here we show that all cell types studied specifically adhered to galectin-3 by the following criteria: 1) the number of adherent cells was dependent on the galectin-3 concentration used for coating; 2) adhesion of cells to galectin-3, but not to collagen type I or laminin was inhibited by polyclonal antibodies to galectin-3; 3) upon addition of asialofetuin (a polyv

Who reads Galectin-3 Promotes Neural Cell Adhesion and Neurite Growth?

Author

Penka Pesheva; Stephan Kuklinski; Brigitte Schmitz; Rainer Probstmeier

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0360-4012)

Published

1998

Language

EN

Field

Neuroscience (Life Sciences)