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Area from image analyses accurately estimates dry‐weight biomass of juvenile tissue from the moss Ceratodon purpureus by Burtscher, Wesley P. (author);List, Marna A. (author);Payton, Adam C. (author);McDaniel, Stuart F. (author);Carey, Sarah B. (author) is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## PREMISE Mosses have long served as models for studying many areas of plant biology. Investigators have used two‐dimensional measurements of juvenile growth from photographs as a surrogate for dry‐weight biomass. The relationship between area and biomass, however, has not been critically evaluated. ## METHODS Here we grew axenic tissue cultures of 10 __Ceratodon purpureus__ isolates to study the relationship between these parameters. We measured area and biomass on replicate cultures with two distinct starting inoculum sizes each week for three weeks. We then examined the correlation between area and biomass as well as the influence of variation in inoculum size on both parameters. ## RESULTS We found a strong correlation between area and biomass after two weeks of growth. Furthermore, we found inoculum size affected biomass during the first week of growth but not in subsequent weeks and inoculum size had no detectable effect on area. ## DISCUSSION These analyses provide experimental confirmation that area is a suitable proxy for biomass and provide clear guidelines for when inoculum size variation may affect downstream growth estimates.

Who reads Area from image analyses accurately estimates dry‐weight biomass of juvenile tissue from the moss Ceratodon purpureus?

Author

Burtscher, Wesley P. (author);List, Marna A. (author);Payton, Adam C. (author);McDaniel, Stuart F. (author);Carey, Sarah B. (author)

Publisher

Wiley

Published

2021

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)