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Molecular analysis of two genes between let-653 and let-56 in the unc 22(IV) region of Caenorhabditis elegans by Marco A. Marra; Shiv S. Prasad; David L. Baillie is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Molecular analysis of two genes between let-653 and let-56 in the unc 22(IV) region of Caenorhabditis elegans about?

A previous study of genomic organization described the identification of nine potential coding regions in 150 kb of genomic DNA from the unc-22(IV) region of Caenorhabditis elegans. In this study, we focus on the genomic organization of a small interval of 0.1 map unit bordered on the right by unc-22 and on the left by the left-hand breakpoints of the deficiencies sDf9, sDf19 and sDf65. This small interval at present contains a single mutagenically defined locus, the essential gene let-56. The cosmid C11F2 has previously been used to rescue let-56. Therefore, at least some of C11F2 must reside in the interval. In this paper, we report the characterization of two coding elements that reside on C11F2. Analysis of nucleotide sequence data obtained from cDNAs and cosmid subclones revealed that one of the coding elements closely resembles aromatic amino acid decarboxylases from several species. The other of these coding elements was found to closely resemble a human growth factor activatable Na+/H+ antiporter. Paris of oligonucleotide primers, predicted from both coding elements, have been used in PCR experiments to position these coding elements between the left breakpoint of sDf19 and

Who reads Molecular analysis of two genes between let-653 and let-56 in the unc 22(IV) region of Caenorhabditis elegans?

Author

Marco A. Marra; Shiv S. Prasad; David L. Baillie

Publisher

Springer; Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC (ISSN 0026-8925)

Published

1993

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)