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Can I read BiP, HSP70, NDK and PDI in wheat endosperm. II. Effects of high temperature on protein and mRNA accumulation on EtoBox?
BiP, HSP70, NDK and PDI in wheat endosperm. II. Effects of high temperature on protein and mRNA accumulation by William J. Hurkman; Frances M. DuPont; Susan B. Altenbach; Allison Combs; Ronald Chan; Charlene K. Tanaka; Moshe Reuveni; John E. Bernardin is a Agricultural and Biological Sciences article available to read on EtoBox.
What is BiP, HSP70, NDK and PDI in wheat endosperm. II. Effects of high temperature on protein and mRNA accumulation about?
The effects of high temperature on accumulation of the 70‐kDa heat shock protein (HSP70) and nucleoside diphosphate kinase (NDK) as well as two other proteins that have roles in the biosynthesis of storage proteins were examined during grain development. An HSP70 homolog and a 17‐kDa NDK were co‐purified from wheat endosperm, their identity verified, and a cDNA for an HSP70 expressed in endosperm was isolated. Wheat plants (__Triticum aestivum__, cvs Butte and Vulcan) were heat shocked at 40°C or exposed to maximum daily temperatures of 37 or 40°C during early or mid‐grain fill. Antibodies and cDNA probes for BiP, HSP70, NDK and PDI were used to examine the effect of high temperatures on the accumulation of protein and mRNA in the endosperm. HSP70 mRNA levels increased substantially when plants were exposed to heat shock or to a 1‐day gradual increase to 40°C. The effects of a 5‐day heat treatment on mRNA levels were more complicated and depended on the developmental stage of the grain. A treatment that began at 7 days post‐anthesis (DPA) decreased the level of mRNA for HSP70, BiP, PDI and NDK, whereas a treatment that began at 14 DPA slightly increased mRNA levels. The same treatm
Who reads BiP, HSP70, NDK and PDI in wheat endosperm. II. Effects of high temperature on protein and mRNA accumulation?
It is typically read by researchers, students, and practitioners in Agricultural and Biological Sciences.
- Author
- William J. Hurkman; Frances M. DuPont; Susan B. Altenbach; Allison Combs; Ronald Chan; Charlene K. Tanaka; Moshe Reuveni; John E. Bernardin
- Publisher
- John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0031-9317)
- Published
- 1998
- Language
- EN
- Field
- Agricultural and Biological Sciences (Life Sciences)