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High-Fat Diet Alters the Expression of Reference Genes in Male Mice by Fan, Xiuqin; Yao, Hongyang; Liu, Xuanyi; Shi, Qiaoyu; Lv, Liang; Li, Ping; Wang, Rui; Tang, Tiantian; Qi, Kemin is a Medicine article available to read on EtoBox.

What is High-Fat Diet Alters the Expression of Reference Genes in Male Mice about?

Quantitative PCR (qPCR), the most accurate and sensitive technique for quantifying mRNA expression, and choice of appropriate reference genes for internal error controlling in qPCR are essential to understanding the molecular mechanisms that drive the obesity epidemic and its comorbidities. In this study, using the high-fat diet (HFD)-induced obese mouse model, we assessed the expression of 10 commonly used reference genes to validate gene-expression stability in adipose tissue, liver, and muscle across different time points (4, 8, 12, and 16 weeks after HFD feeding) during the process of obesity. The data were analyzed by the GeNorm, NormFinder, BestKeeper, and Delta-Ct method, and the results showed that the most stable reference genes were different for a specific organ or tissue in a specific time point; however, PPIA, RPLP0, and YWHAZ were the top three most stable reference genes in qPCR experiments on adipose, hepatic tissues, and muscles of mice in diet-induced obesity. In addition, the mostly used genes ACTB and GAPDH were more unstable in the fat and liver, the ACTB mRNA levels were increased in four adipose tissues, and the GAPDH mRNA levels were decreased in four adipos

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Author

Fan, Xiuqin; Yao, Hongyang; Liu, Xuanyi; Shi, Qiaoyu; Lv, Liang; Li, Ping; Wang, Rui; Tang, Tiantian; Qi, Kemin

Publisher

Frontiers Media SA; Frontiers Media S.A.; Lausanne: Frontiers Media S.A., 2014- (ISSN 2296-861X)

Published

2020

Language

EN

Field

Medicine (Health Sciences)