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Noninvasive Measurements of [1‐^13^C] Glycogen Concentrations and Metabolism in Rat Brain In Vivo by In-Young Choi; Ivan Tkáčc; Kâmil Ugurbil; Rolf Gruetter is a Neuroscience article available to read on EtoBox.

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Abstract : Using a specific ^13^C NMR localization method, ^13^C label incorporation into the glycogen C1 resonance was measured while infusing [1‐^13^C]glucose in intact rats. The maximal concentration of [1‐^13^C]glycogen was 5.1 ± 0.6 μmol g^‐1^ (mean ± SE, n = 8). During the first 60 min of acute hyperglycemia, the rate of ^13^C label incorporation (synthase flux) was 2.3 ± 0.7 μmol g^‐1^ h^‐1^ (mean ± SE, n = 9 rats), which was higher (__p__ <0.01) than the rate of 0.49 ± 0.14 μmol g^‐1^ h^‐1^ measured ≥2 h later. To assess whether the incorporation of ^13^C label was due to turnover or net synthesis, the infusion was continued in seven rats with unlabeled glucose. The rate of ^13^C label decline (phosphorylase flux) was lower (0.33 ± 0.10 μmol g^‐1^ h^‐1^) than the initial rate of label incorporation (__p__ 0.05 for correlation). The results implied that net glycogen synthesis of ~3 μmol g^‐1^ had occurred, similar to previous reports. When infusing unlabeled glucose before [1‐^13^C]glucose in three studies, the rate of glycogen C1 accumulation was 0.46 ± 0.08 μmol g^‐1^ h^‐1^. The results suggest that steady‐state glycogen turnover rates during hyperglycemia are ~1% of glu

Who reads Noninvasive Measurements of [1‐^13^C] Glycogen Concentrations and Metabolism in Rat Brain In Vivo?

Author

In-Young Choi; Ivan Tkáčc; Kâmil Ugurbil; Rolf Gruetter

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0022-3042)

Published

1999

Language

EN

Field

Neuroscience (Life Sciences)