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Dynamically Softened Substrate Regulates Malignancy of Breast Tumor Cells by Mi Hu; Xu Li; Wei-Pin Huang; Deng-Feng Hu; Ke-Feng Ren; Jian Ji is a Engineering article available to read on EtoBox.

What is Dynamically Softened Substrate Regulates Malignancy of Breast Tumor Cells about?

It has long been hypothesized that an increase in the extracellular matrix (ECM) stiffness mechanoactivates malignant phenotypes of breast tumor cells by regulating an array of processes underlying cancer biology. Although the contribution of substrate stiffening to drive malignant phenotype traits and other biological functions of a tumor is increasingly understood, the functional role of substrate softening on breast cancer cellular responses has rarely been investigated. Herein, we employed matrix metalloproteinase (MMP)-sensitive film to perform assays to explore the consequences of lowering stiffness on the biological behaviors of breast cancer cell MDA-MB-231. We demonstrated that cells underwent dramatic changes in migration, cellular conjunction, and expression of malignance-associated proteins and genes when the substrate stiffness decreased. Based on RNA sequencing and analysis, we found that hub genes including TP53, CCND1, MYC, CTNNB1, and YAP were validated to play central parts in regulating stiffness-dependent cellular manner change. Moreover, through visualization of differentially expressed genes (DEGs), cells on dynamically softened substrate appeared less influen

Who reads Dynamically Softened Substrate Regulates Malignancy of Breast Tumor Cells?

Author

Mi Hu; Xu Li; Wei-Pin Huang; Deng-Feng Hu; Ke-Feng Ren; Jian Ji

Publisher

Springer Science and Business Media LLC

Published

2021

Language

EN

Field

Engineering (Physical Sciences)