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Nitric oxide regulates axonal regeneration in an insect embryonic CNS by Michael Stern; Gerd Bicker is a Neuroscience article available to read on EtoBox.

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## Abstract In higher vertebrates, the central nervous system (CNS) is unable to regenerate after injury, at least partially because of growth‐inhibiting factors. Invertebrates lack many of these negative regulators, allowing us to study the positive factors in isolation. One possible molecular player in neuronal regeneration is the nitric oxide (NO)—cyclic guanosine‐monophosphate (cGMP) transduction pathway which is known to regulate axonal growth and neural migration. Here, we present an experimental model in which we study the effect of NO on CNS regeneration in flat‐fillet locust embryo preparations in culture after crushing the connectives between abdominal ganglia. Using whole‐mount immunofluorescence, we examine the morphology of identified serotonergic neurons, which send a total of four axons through these connectives. After injury, these axons grow out again and reach the neighboring ganglion within 4 days in culture. We quantify the number of regenerating axons within this period and test the effect of drugs that interfere with NO action. Application of exogenous NO or cGMP promotes axonal regeneration, whereas scavenging NO or inhibition of soluble guanylyl cyclase dela

Who reads Nitric oxide regulates axonal regeneration in an insect embryonic CNS?

Author

Michael Stern; Gerd Bicker

Publisher

Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1932-8451)

Published

2007

Language

EN

Field

Neuroscience (Life Sciences)