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Carbohydrate-specific Adhesion of Alveolar Macrophages to Mannose-derivatized Surfaces. by B L Largent; K M Walton; C A Hoppe; Y C Lee; R L Schnaar is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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The ability of rabbit alveolar macrophages to specifically recognize and adhere to surfaces derivatized with carbohydrates was examined. Otherwise inert polyacrylamide gels were derivatized with aminohexylglycosides as previously described (Guarnaccia, S. P., and Schnaar, R. L. (1982) J. Biol. Chem. 257, 14288-14292). Intact viable rabbit alveolar macrophages, isolated by lung lavage, were placed in contact with surfaces derivatized with different glycosides. Only those surfaces derivatized with a-D-mannose residues were capable of supporting rabbit alveolar macrophage adhesion. Adhesion was rapid, obtaining maximal levels within 10 min, and occurred readily at either 0 or 37 "C. The carbohydrate specificity of the cell adhesion was investigated by the use of soluble carbohydrate inhibitors. The potency of various saccharides to block the adhesion correlated with that demonstrated for blocking the uptake or binding of radiolabeled soluble glycoproteins (Shepherd,

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Author

B L Largent; K M Walton; C A Hoppe; Y C Lee; R L Schnaar

Publisher

Elsevier BV

Published

1984

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)