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Increased copy number at 17q22-q24 by CGH in breast cancer is due to high-level amplification of two separate regions by Maarit Bärlund; Mika Tirkkonen; Farahnaz Forozan; Minna M. Tanner; Olli Kallioniemi; Anne Kallioniemi is a Medicine article available to read on EtoBox.
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Studies by comparative genomic hybridization (CGH) have defined a chromosomal site at 17q22-q24 that is often overrepresented in breast cancer, neuroblastoma, and several other tumor types. Due to the limited resolution and dynamic range of CGH, it remains unclear whether this gain reflects high-level amplification of small subregion(s) or low-level gain of most of the distal 17q. We used 32 physically mapped 17q probes to construct more accurate copy number profiles for 14 breast cancer cell lines by interphase fluorescence in situ hybridization (FISH). Six cell lines (43%) showed an increased copy number of the 17q22-q24 region by CGH, and seven (50%) by FISH. FISH copy number profiles had a substantially higher dynamic range than did CGH profiles. FISH revealed two independent, highly amplified regions (A and B) at 17q23, separated by about 5 Mb of non-amplified DNA. These regions were distinctly telomeric from the ERBB2 gene locus. However, region A was often co-amplified with ERBB2, whereas B was amplified in cell lines that showed no ERBB2 amplification. We conclude that distal 17q gains recently discovered in breast cancer by CGH are due to high-level amplifications of two d
Who reads Increased copy number at 17q22-q24 by CGH in breast cancer is due to high-level amplification of two separate regions?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Maarit Bärlund; Mika Tirkkonen; Farahnaz Forozan; Minna M. Tanner; Olli Kallioniemi; Anne Kallioniemi
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); Wiley-Liss Inc; Wiley (ISSN 1045-2257)
- Published
- 1997
- Language
- EN
- Field
- Medicine (Life Sciences)