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Polarizing microscopy of eyespot of Chlamydomonas: In situ observation of its location, orientation, and multiplication by Shi-Yuan Yang; Masamichi Tsuboi is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Polarizing microscopy of eyespot of Chlamydomonas: In situ observation of its location, orientation, and multiplication about?

The eyespot in the cell of Chlamydomonas reinhardtii has been found to appear as a bright spot under the cross polar setting of a polarizing microscope. This was confirmed by isolating the eyespot from a homogenate of wall-deficient mutant cw-15, and by observing it under a polarizing microscope. Thus, the eyespot was proved to be a strongly birefringent body. Next, gametes (mt+ and mt-) of 137c strain were prepared by cultivating it in a low-nitrogen (NH4Cl) medium. Here, every cell shows only one (and never more than one) birefringent spot. The birefringent eyespot was located always near the surface on the "equator," that is, at the farthest point from the "meridionial" cell-axis that is defined as the bisector of the two flagella projected out from the cell surface. It was shown, in addition, that the optic axis of this birefringent eyespot is oriented in the cell always along the parallel direction of the cell axis defined above. Thus, the polarizing microscopy has been shown to provide a powerful method for in vivo, in situ pursuit of the eyespot of Chlamydomonas.

Who reads Polarizing microscopy of eyespot of Chlamydomonas: In situ observation of its location, orientation, and multiplication?

Author

Shi-Yuan Yang; Masamichi Tsuboi

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1075-4261)

Published

1999

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)