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Monoclonal Antibody Against Adult Marrow-derived Mesenchymal Stem Cells Recognizes Developing Vasculature in Embryonic Human Skin by James E. Fleming Jr.; Stephen E. Haynesworth; Pierre Cassiede; Marilyn A. Baber; Arnold I. Caplan is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Monoclonal Antibody Against Adult Marrow-derived Mesenchymal Stem Cells Recognizes Developing Vasculature in Embryonic Human Skin about?

We have described previously a monoclonal antibody (SH2) that specifically recognizes undifferentiated mesenchymal progenitor cells isolated from adult human bone marrow. These cells, which we operationally refer to as mesenchymal stem cells, have the capacity to differentiate and form distinct mesenchymal tissues such as bone and cartilage when the isolated cells are placed in the appropriate in vivo or in vitro environment. We report here the partial biochemical characterization of the antigen recognized by the SH2 antibody. Metabolically radiolabelled adult marrow-derived mesenchymal stem cells in culture were extracted and immunoprecipitated with the SH2 antibody. The purified antigen migrated as a single band of 90 kDa after sodium dodecyl sulfate polyacrylamide gel electrophoresis was performed under reducing conditions. The SH2-immunoprecipitated protein exhibited a molecular weight band shift after removal of N-linked oligosaccharides. We investigated the expression of the SH2 antigen, along with the endothelial markers factor VIII-related antigen and Ulex europaeus I (UEA-I) lectin during specific developmental periods in human dermal embryogenesis and in the postnatal per

Who reads Monoclonal Antibody Against Adult Marrow-derived Mesenchymal Stem Cells Recognizes Developing Vasculature in Embryonic Human Skin?

Author

James E. Fleming Jr.; Stephen E. Haynesworth; Pierre Cassiede; Marilyn A. Baber; Arnold I. Caplan

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1058-8388)

Published

1998

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)