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Determination of Ethambutol in Plasma by High-performance Liquid Chromatography After Pre-column Derivatization by Philippe Chenevier; Laurent Massias; Delphine Gueylard; Robert Farinotti is a Chemistry article available to read on EtoBox.

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A new HPLC assay using UV detection (200 nm) was developed to determine ethambutol (EMB) concentrations in plasma. Following extraction (0.1 ml plasma) with chloroform, EMB and octylamine (used as internal standard) were derivatized with phenylethylisocyanate. Quantitation in plasma was achieved at 200 nm. There were no interferences from endogenous compounds. Intra-and inter-day variabilities were lower than 5.2 and 7.6%, respectively. The limit of quantitation of the method was 0.2 mg / ml. In plasma, ethambutol was found to be stable for at least one month when samples were stored at 2208C. This assay was applied to the therapeutic monitoring of EMB concentrations in 19 patients suffering from tuberculosis.

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Author

Philippe Chenevier; Laurent Massias; Delphine Gueylard; Robert Farinotti

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0378-4347)

Published

1998

Language

EN

Field

Chemistry (Physical Sciences)