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Can I read Development of novel fluorescence-based and label-free noncanonical G4-quadruplex-like DNA biosensor for facile, specific, and ultrasensitive detection of fipronil on EtoBox?
Development of novel fluorescence-based and label-free noncanonical G4-quadruplex-like DNA biosensor for facile, specific, and ultrasensitive detection of fipronil by Kien Hong Trinh; Ulhas Sopanrao Kadam; Shailima Rampogu; Yuhan Cho; Kyung-Ae Yang; Chang Ho Kang; Keun-Woo Lee; Kyun Oh Lee; Woo Sik Chung; Jong Chan Hong is a Environmental Science article available to read on EtoBox.
What is Development of novel fluorescence-based and label-free noncanonical G4-quadruplex-like DNA biosensor for facile, specific, and ultrasensitive detection of fipronil about?
Fipronil is a broad-spectrum insecticide widely used in agriculture and residential areas; its indiscriminate use leads to environmental pollution and poses health hazards. Early detection of fipronil is critical to prevent the deleterious effects. However, current insecticide analysis methods such as HPLC, LC/MS, and GC/MS are incompetent; they are costly, immobile, time-consuming, laborious, and need skilled technicians. Hence, a sensitive, specific, and cheap biosensor are essential to containing the contamination. Here, we designed two novel biosensors-the first design relied on fluorescent labeling/quenching, while the second sensor focused on label-free detection using Thioflavin T displacement. Altogether, we identified four candidate aptamers, predicted secondary structures, and performed 3D molecular modeling to predict the binding pocket of fipronil in FiPA6B aptamer. Furthermore, the aptameric sensors showed high sensitivity to fipronil of sub-ppb level LOD, attributed to stringent experimental design. The biosensors displayed high specificity against other phenylpyrazole insecticides and demonstrated robust sensitivity for fipronil in real samples like cabbage and cucum
Who reads Development of novel fluorescence-based and label-free noncanonical G4-quadruplex-like DNA biosensor for facile, specific, and ultrasensitive detection of fipronil?
It is typically read by researchers, students, and practitioners in Environmental Science.
- Author
- Kien Hong Trinh; Ulhas Sopanrao Kadam; Shailima Rampogu; Yuhan Cho; Kyung-Ae Yang; Chang Ho Kang; Keun-Woo Lee; Kyun Oh Lee; Woo Sik Chung; Jong Chan Hong
- Publisher
- Elsevier BV
- Published
- 2022
- Language
- EN
- Field
- Environmental Science (Physical Sciences)