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Can I read Near-infrared, dual-ratiometric fluorescent label for measurement of pH on EtoBox?
Near-infrared, dual-ratiometric fluorescent label for measurement of pH by Yevgen A. Povrozin; Larysa I. Markova; Anatoliy L. Tatarets; Vadim I. Sidorov; Ewald A. Terpetschnig; Leonid D. Patsenker is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Near-infrared, dual-ratiometric fluorescent label for measurement of pH about?
We describe the spectral properties of an amine-reactive, pH-sensitive, long-wavelength ratiometric fluorescent label having a pK a in the physiological pH range. The label exhibits its main absorption and emission in the near-infrared (NIR) region. On deprotonation, a blue shift of the excitation maximum is observed. Importantly, both the protonated and deprotonated forms of the label are fluorescent, with the deprotonated form having an extremely large Stokes shift of more than 100 nm. The spectral and photophysical properties of this pH label are compared with the properties of the protein-conjugated forms. Due to the observed pK a shift to the acidic pH range upon conjugation to proteins, such labels are ideal for studying phagocytic events and their regulation by drugs and/or environmental factors.
Who reads Near-infrared, dual-ratiometric fluorescent label for measurement of pH?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Yevgen A. Povrozin; Larysa I. Markova; Anatoliy L. Tatarets; Vadim I. Sidorov; Ewald A. Terpetschnig; Leonid D. Patsenker
- Publisher
- Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 0003-2697)
- Published
- 2009
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)