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Glycerol Stock Preparation SOP for Bacteria by ashra sindhikkaa is a document available to read on EtoBox.
This standard operating procedure outlines two methods for preserving bacterial isolates and control strains - glycerol stocks for long term storage below -20°C, and agar stabs for short term storage up to 1-2 years at room temperature. It describes preparing 30% glycerol solution and 15% glycerol stocks of mid-log phase cultures for freezing. It also provides instructions for preparing agar deeps in screw capped tubes, stab inoculating with overnight cultures, and labeling and storing the agar stabs at roo
- Author
- ashra sindhikkaa
- Language
- EN