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Immunohistochemical and biochemical analysis of serotonin and substance P colocalization in the nucleus tractus solitarii and associated afferent ganglia of the rat by Karl B. Thor; Kelly M. Hill; Christelle Harrod; Cinda J. Helke is a Neuroscience article available to read on EtoBox.

What is Immunohistochemical and biochemical analysis of serotonin and substance P colocalization in the nucleus tractus solitarii and associated afferent ganglia of the rat about?

In a previous study of afferent projections to the nucleus tractus solitarii (NTS), it was shown that over half of the retrogradely-labelled neurons in the nucleus raphe pallidus contained serotonin-immunoreactivity and over half of these neurons contained substance P-immunoreactivity , suggesting that these two putative neurotransmitters are colocalized in NTS-afferent neurons. The objectives of the present study were to 1) directly determine if varicosities in the NTS, the area postrema (AP), and the dorsal motor nucleus of the vagus nerve (DMN) do contain both transmitters, 2) determine if primary afferent neurons in the nodose and petrosal ganglia might also colocalize serotonin and substance P, and 3) quantify the amount of substance P that is contained in serotonergic varicosities in the NTS. Distributions and colocalization of substance P and serotonin in the NTS were studied using dual-color immunohistochemistry, while the quantity of substance P in serotonergic varicosities was assessed by radioimmunoassay (RIA) using micropunches from the NTS of 5 ,?-dihydroxytryptamine-(5,7 DHT-) and vehicle-treated rats. Varicosities that contained both serotonin-and substance P-immunor

Who reads Immunohistochemical and biochemical analysis of serotonin and substance P colocalization in the nucleus tractus solitarii and associated afferent ganglia of the rat?

Author

Karl B. Thor; Kelly M. Hill; Christelle Harrod; Cinda J. Helke

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0887-4476)

Published

1987

Language

EN

Field

Neuroscience (Life Sciences)