Can I read Bienzyme Amperometric Biosensor Using Gold Nanoparticle-modified Electrodes for the Determination of Inulin in Foods on EtoBox?

Bienzyme Amperometric Biosensor Using Gold Nanoparticle-modified Electrodes for the Determination of Inulin in Foods by Javier Manso; Ma Luz Mena; P. Yáñez-Sedeño; José M. Pingarrón is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Bienzyme Amperometric Biosensor Using Gold Nanoparticle-modified Electrodes for the Determination of Inulin in Foods about?

A biosensor design involving coimmobilization of fructose dehydrogenase (FDH) and inulinase (INU) on a gold nanoparticle-cysteamine (Cyst) self-assembled monolayer (SAM)-modified gold electrode (Au(coll)-Cyst-AuE), for the determination of the carbohydrate inulin in foodstuffs, is reported. Tetrathiafulvalene (TTF), used as the mediator, was also coimmobilized by crosslinking with glutaraldehyde. INU catalyzes the hydrolysis of inulin, forming fructose that is detected through the fructose dehydrogenase system by the electrochemical oxidation of TTF at the bioelectrode. The variables involved in the preparation and performance of both the single enzyme FDH biosensor and the bienzyme inulin biosensor were optimized. The FDH-Au(coll)-Cyst-AuE biosensor exhibited rapid and sensitive response to fructose, allowing the obtention of improved analytical characteristics for the determination of fructose with respect to other FDH electrochemical biosensors. Moreover, the lifetime of this biosensor was 35 days. The bienzyme INU/FDH-Au(coll)-Cyst-AuE biosensor provided a calibration plot for inulin in the (5-100)x10(-6) M linear range, with a detection limit of 6.6 x 10(-7) mol L(-1). One sin

Who reads Bienzyme Amperometric Biosensor Using Gold Nanoparticle-modified Electrodes for the Determination of Inulin in Foods?

Author

Javier Manso; Ma Luz Mena; P. Yáñez-Sedeño; José M. Pingarrón

Publisher

Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 0003-2697)

Published

2008

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)