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A cyanobacterial narB gene encodes a ferredoxin-dependent nitrate reductase by Luis M. Rubio; Antonia Herrero; Enrique Flores is a Agricultural and Biological Sciences article available to read on EtoBox.

What is A cyanobacterial narB gene encodes a ferredoxin-dependent nitrate reductase about?

The narB gene from the cyanobacterium Synechococcus sp. PCC 7942 was cloned downstream from the LacI-regulated promoter P,rc in the Escherichia coli vector pTrc99A, rendering plasmid p C S L M 1. Addition of isopropyl-~-D-thiogalactoside to E. coli (pCSLM1) resulted in the parallel expression of a 76 kDa polypeptide and a nitrate reductase activity with properties identical to those known for nitrate reductase isolated from Synechococcus cells. As is the case for nitrate reductase from Synechococcus cells, either reduced methyl viologen or reduced ferredoxin could be used as an electron donor for the reduction of nitrate catalyzed by E. coli (pCSLM1) extracts. This data shows that narB is a cyanobacterial structural gene for nitrate reductase.

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Author

Luis M. Rubio; Antonia Herrero; Enrique Flores

Publisher

Springer; Springer-Verlag; Kluwer Academic Publishers; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 0167-4412)

Published

1996

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)