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Foreword 0 1 Scope 0 2 Terms and definitions 0 3 Principle 0 4 Reagents and materials 0 5 Apparatus 0 6 Procedure 0 6.1 General 0 6.2 Sample preparation 0 6.2.1 General 0 6.2.2 Extraction 0 6.2.3 Sample concentration 0 6.3 HPLC analysis 0 6.3.1 System setup and configuration 0 6.3.2 Instrument operation conditions 0 7 Calculations 0 7.1 General 0 7.2 Calculation of standard solution concentrations 0 7.3 Preparation of standard curves 0 7.4 Calculation of vitamin B12 concentrations in sample solutions 0 Annex A (informative) Examples of chromatograms 0 Annex B (informative) Precision data 0 Annex C (informative) Example of SPE cartridge qualification procedure 0 Bibliography 0

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