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Mesenchymal Stromal Cells in the Bone Marrow Niche Consist of Multi-populations with Distinct Transcriptional and Epigenetic Properties by Kanazawa, Sanshiro (author);Okada, Hiroyuki (author);Hojo, Hironori (author);Ohba, Shinsuke (author);Iwata, Junichi (author);Komura, Makoto (author);Hikita, Atsuhiko (author);Hoshi, Kazuto (author) is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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Although multiple studies have investigated the mesenchymal stem and progenitor cells (MSCs) that give rise to mature bone marrow, high heterogeneity in their morphologies and properties causes difficulties in molecular separation of their distinct populations. In this study, by taking advantage of the resolution of the single cell transcriptome, we analyzed Sca-1 and PDGFR-α fraction in the mouse bone marrow tissue. The single cell transcriptome enabled us to further classify the population into seven populations according to their gene expression profiles. We then separately obtained the seven populations based on candidate marker genes, and specified their gene expression properties and epigenetic landscape by ATAC-seq. Our findings will enable to elucidate the stem cell niche signal in the bone marrow microenvironment, reconstitute bone marrow in vitro, and shed light on the potentially important role of identified subpopulation in various clinical applications to the treatment of bone- and bone marrow-related diseases.

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Author

Kanazawa, Sanshiro (author);Okada, Hiroyuki (author);Hojo, Hironori (author);Ohba, Shinsuke (author);Iwata, Junichi (author);Komura, Makoto (author);Hikita, Atsuhiko (author);Hoshi, Kazuto (author)

Publisher

Springer Science and Business Media LLC

Published

2021

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)