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Diagnosis of human leptospirosis: systematic review and meta-analysis of the diagnostic accuracy of the Leptospira microscopic agglutination test, PCR targeting Lfb1 , and IgM ELISA to Leptospira fainei serovar Hurstbridge by Marta Valente; Justina Bramugy; Suzanne H. Keddie; Heidi Hopkins; Quique Bassat; Oliver Baerenbold; John Bradley; Jane Falconer; Ruth H. Keogh; Paul N. Newton; Mathieu Picardeau; John A. Crump is a Medicine article available to read on EtoBox.

What is Diagnosis of human leptospirosis: systematic review and meta-analysis of the diagnostic accuracy of the Leptospira microscopic agglutination test, PCR targeting Lfb1 , and IgM ELISA to Leptospira fainei serovar Hurstbridge about?

## Background Leptospirosis is an underdiagnosed infectious disease with non-specific clinical presentation that requires laboratory confirmation for diagnosis. The serologic reference standard remains the microscopic agglutination test (MAT) on paired serum samples. However, reported estimates of MAT’s sensitivity vary. We evaluated the accuracy of four index tests, MAT on paired samples as well as alternative standards for leptospirosis diagnosis: MAT on single acute-phase samples, polymerase chain reaction (PCR) with the target gene __Lfb1__, and ELISA IgM with __Leptospira fainei__ serovar Hurstbridge as an antigen. ## Methods We performed a systematic review of studies reporting results of leptospirosis diagnostic tests. We searched eight electronic databases and selected studies that tested human blood samples and compared index tests with blood culture and/or PCR and/or MAT (comparator tests). For MAT selection criteria we defined a threshold for single acute-phase samples according to a national classification of leptospirosis endemicity. We used a Bayesian random-effect meta-analysis to estimate the sensitivity and specificity of MAT in single acute-phase and paired sample

Who reads Diagnosis of human leptospirosis: systematic review and meta-analysis of the diagnostic accuracy of the Leptospira microscopic agglutination test, PCR targeting Lfb1 , and IgM ELISA to Leptospira fainei serovar Hurstbridge?

Author

Marta Valente; Justina Bramugy; Suzanne H. Keddie; Heidi Hopkins; Quique Bassat; Oliver Baerenbold; John Bradley; Jane Falconer; Ruth H. Keogh; Paul N. Newton; Mathieu Picardeau; John A. Crump

Publisher

BioMed Central

Published

2024

Language

EN

Field

Medicine (Health Sciences)