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Serum epitope repertoire analysis enables early detection of Lyme disease with improved sensitivity in an expandable multiplex format by Reifert, Jack; Kamath, Kathy; Bozekowski, Joel; Lis, Ewa; Horn, Elizabeth J.; Granger, Dane; Theel, Elitza S.; Shon, John; Sawyer, Jaymie R.; Daugherty, Patrick S. is a Medicine article available to read on EtoBox.

What is Serum epitope repertoire analysis enables early detection of Lyme disease with improved sensitivity in an expandable multiplex format about?

Widely employed diagnostic antibody serology for Lyme disease, known as standard two-tier testing (STTT), exhibits insufficient sensitivity in early Lyme disease, yielding many thousands of false-negative test results each year. Given this problem, we applied serum antibody repertoire analysis (SERA), or next-generation sequencing (NGS)-based serology, to discover IgG and IgM antibody epitope motifs capable of detecting Lyme disease-specific antibodies with high sensitivity and specificity. Iterative motif discovery and bioinformatic analysis of epitope repertoires from subjects with Lyme disease (__n__ = 264) and controls (__n__ = 391) yielded a set of 28 epitope motifs representing 20 distinct IgG antibody epitopes and a set of 38 epitope motifs representing 21 distinct IgM epitopes, which performed equivalently in a large validation cohort of STTT-positive samples.

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Author

Reifert, Jack; Kamath, Kathy; Bozekowski, Joel; Lis, Ewa; Horn, Elizabeth J.; Granger, Dane; Theel, Elitza S.; Shon, John; Sawyer, Jaymie R.; Daugherty, Patrick S.

Publisher

American Society for Microbiology (ISSN 0095-1137)

Published

2021

Language

EN

Field

Medicine (Health Sciences)