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Protein kinase C involvement in the resting and interferon-γ-induced K+ channel profile of microglial cells by Sergio Visentin; Giulio Levi is a Neuroscience article available to read on EtoBox.

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The whole-cell configuration of the patch-clamp technique was used to study the involvement of protein kinase C (PKC) in the modulation of K 1 channels in cultured microglia from newborn rats. We previously showed that 24-hr treatments with interferon-g (IFN-g) induce an increase of inward-rectifying (IR) and outward-rectifying (OR) current density and that the effect on OR was shared by bacterial lipopolysaccharide (LPS) (Visentin et al.: J Neurosci Res 42:439-451, 1995). In the present study, IFN-g (1-500 U/ml, 24 hr) enhanced IR current density up to threefold. The IFN-g effect was not detectable after shorter treatments (1-5 hr) and was abrogated by a protein synthesis inhibitor. The PKC activator phorbol myristate acetate (PMA) also increased IR current density, whereas the inactive a-4 isoform was ineffective. When IFN-g and PMA were co-applied, the effect was more than additive. Among the PKC inhibitors tested, staurosporine (STA)-but not calphostin C (CALP)abolished the effect of IFN-g and of PMA and antagonized only partially that of co-applied IFN-g and PMA. OR currents were affected by treatment (24 hr) with PKC modulating agents in an opposite fashion. PMA depressed OR

Who reads Protein kinase C involvement in the resting and interferon-γ-induced K+ channel profile of microglial cells?

Author

Sergio Visentin; Giulio Levi

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0360-4012)

Published

1997

Language

EN

Field

Neuroscience (Life Sciences)