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Inter- and Intra-Species Diversity of Lactic Acid Bacteria in Apis mellifera ligustica Colonies by Iorizzo, Massimo; Pannella, Gianfranco; Lombardi, Silvia Jane; Ganassi, Sonia; Testa, Bruno; Succi, Mariantonietta; Sorrentino, Elena; Petrarca, Sonia; De Cristofaro, Antonio; Coppola, Raffaele; Tremonte, Patrizio is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Inter- and Intra-Species Diversity of Lactic Acid Bacteria in Apis mellifera ligustica Colonies about?

Lactic acid bacteria could positively affect the health of honey bees, including nutritional supplementation, immune system development and pathogen colonization resistance. Based on these considerations the present study evaluated predominant Lactic Acid Bacteria (LAB) species from beebread as well as from the social stomach and midgut of Apis mellifera ligustica honey bee foragers. In detail, for each compartment, the diversity in species and biotypes was ascertained through multiple culture-dependent approaches, consisting of Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis (PCR-DGGE), 16S rRNA gene sequencing and Randomly Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR). The study of a lactic acid bacteria community, performed with PCR-DGGE and sequence analysis targeting the V1–V3 region of the 16S rRNA gene (rDNA), highlighted the presence of a few species, including Apilactobacillus kunkeei, Lactiplantibacillus plantarum, Fructobacillus fructosus, Levilactobacillus brevis and Lactobacillus delbrueckii subsp. lactis. Depending on the different compartments, diverse levels of biodiversity in species were found. Particularly, a very low inter-spec

Who reads Inter- and Intra-Species Diversity of Lactic Acid Bacteria in Apis mellifera ligustica Colonies?

It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.

Author
Iorizzo, Massimo; Pannella, Gianfranco; Lombardi, Silvia Jane; Ganassi, Sonia; Testa, Bruno; Succi, Mariantonietta; Sorrentino, Elena; Petrarca, Sonia; De Cristofaro, Antonio; Coppola, Raffaele; Tremonte, Patrizio
Publisher
Multidisciplinary Digital Publishing Institute (MDPI) (ISSN 2076-2607)
Published
2020
Language
EN
Field
Biochemistry, Genetics and Molecular Biology (Life Sciences)

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