Opening book details…
Can I read Sequence Comparison of the EcoHK31I and EaeI. Restriction-Modification Systems Suggests an Intergenic Transfer of Genetic Material on EtoBox?
Sequence Comparison of the EcoHK31I and EaeI. Restriction-Modification Systems Suggests an Intergenic Transfer of Genetic Material by Kai-Fai Lee; Pang-Chui Shaw; Steven J. Picone; Geoffrey G. Wilson; Keith D. Lunnen is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Sequence Comparison of the EcoHK31I and EaeI. Restriction-Modification Systems Suggests an Intergenic Transfer of Genetic Material about?
The genes coding for the EcoHK31l and Eael restriction-modification (R-M) systems from Escherichia coll strain HK31 and Enterobacter aerogenes, respectively, have been cloned and sequenced. Both ENases recognize and cleave Y|GGCCR leaving 4 nucleotide 5'-protruding ends, while the MTases modify the internal cytosine. The systems were isolated on a 2.3kb Ase\ fragment for EcoHK311, and a 4.6 kb H/ndlll fragment for Eael. The R and M genes of both systems converge and overlap by 14 nucleotides. Previously, we found that M.EcoHK311 consisted of two subunits, (a and β), with the β subunit being translated from an alternative open reading frame within the gene encoding the α subunit. Sequence comparison between the EcoHK311 and Eael systems reveals striking similarity. The eaelM gene also encodes α and β polypeptides of 309 and 176 amino acids which share 96% and 97% identity, respectively, with those of ecoHK31IM. ecoHK31 IR and eaeIR encode proteins of 318 and 315 aa, respectively, which share 92% identity but are otherwise unique in the GenBank database. The Eael and the EcoH K311 R-M systems were found to be flanked by genes coding for integrases. It is possible that these integrase
Who reads Sequence Comparison of the EcoHK31I and EaeI. Restriction-Modification Systems Suggests an Intergenic Transfer of Genetic Material?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Kai-Fai Lee; Pang-Chui Shaw; Steven J. Picone; Geoffrey G. Wilson; Keith D. Lunnen
- Published
- 1998
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)