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Doxorubicin Treatment Modulates Chemoresistance and Affects the Cell Cycle in Two Canine Mammary Tumour Cell Lines by Levi, Michela (author);Salaroli, Roberta (author);Parenti, Federico (author);De Maria, Raffaella (author);Zannoni, Augusta (author);Bernardini, Chiara (author);Gola, Cecilia (author);Brocco, Antonio (author);Marangio, Asia (author);Benazzi, Cinzia (author);Muscatello, Luisa Vera (author);Brunetti, Barbara (author);Forni, Monica (author);Sarli, Giuseppe (author) is a Agricultural and Biological Sciences article available to read on EtoBox.

What is Doxorubicin Treatment Modulates Chemoresistance and Affects the Cell Cycle in Two Canine Mammary Tumour Cell Lines about?

## Abstract ## Background Doxorubicin (DOX) is widely used in both human and veterinary oncology although the onset of multidrug resistance (MDR) in neoplastic cells often leads to chemotherapy failure. Better understanding of the cellular mechanisms that circumvent chemotherapy efficacy is paramount. The aim of this study was to investigate the response of two canine mammary tumour cell lines, CIPp from a primary tumour and CIPm, from its lymph node metastasis, to exposure to EC50~(20h)~ DOX at 12, 24 and 48 h of treatment. We assessed the uptake and subcellular distribution of DOX, the expression and function of P-glycoprotein (P-gp) and Breast Cancer Resistance Protein (BCRP), two important MDR mediators. To better understand this phenomenon the effects of DOX on the cell cycle and Ki67 cell proliferation index and the expression of p53 and telomerase reverse transcriptase (TERT) were also evaluated by immunocytochemistry (ICC). ## Results Both cell lines were able to uptake DOX within the nucleus at 3 h treatment while at 48 h DOX was absent from the intracellular compartment (assessed by fluorescence microscope) in all the surviving cells. CIPm, originated from the metastatic

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Author

Levi, Michela (author);Salaroli, Roberta (author);Parenti, Federico (author);De Maria, Raffaella (author);Zannoni, Augusta (author);Bernardini, Chiara (author);Gola, Cecilia (author);Brocco, Antonio (author);Marangio, Asia (author);Benazzi, Cinzia (author);Muscatello, Luisa Vera (author);Brunetti, Barbara (author);Forni, Monica (author);Sarli, Giuseppe (author)

Publisher

Springer Science and Business Media LLC

Published

2021

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)