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Can I read Cell Position Within Human Pluripotent Stem Cell Colonies Determines Apical Specialization via an Actin Cytoskeleton-based Mechanism on EtoBox?
Cell Position Within Human Pluripotent Stem Cell Colonies Determines Apical Specialization via an Actin Cytoskeleton-based Mechanism by Kim, Youngju (author);Jang, Hwanseok (author);Seo, Kyubin (author);Kim, June Hoan (author);Lee, Boram (author);Cho, Hyo Min (author);Kim, Hyun Jung (author);Yang, Esther (author);Kim, Hyun (author);Gim, Jeong-An (author);Park, Yongdoo (author);Ryu, Jae Ryun (author);Sun, Woong (author) is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Cell Position Within Human Pluripotent Stem Cell Colonies Determines Apical Specialization via an Actin Cytoskeleton-based Mechanism about?
## Summary Human pluripotent stem cells (hPSCs) grow as colonies with epithelial-like features including cell polarity and position-dependent features that contribute to symmetry breaking during development. Our study provides evidence that hPSC colonies exhibit position-dependent differences in apical structures and functions. With this apical difference, edge cells were preferentially labeled with amphipathic dyes, which enabled separation of edge and center cells by fluorescence-activated cell sorting. Transcriptome comparison between center and edge cells showed differential expression of genes related to apicobasal polarization, cell migration, and endocytosis. Accordingly, different kinematics and mechanical dynamics were found between center and edge cells, and perturbed actin dynamics disrupted the position-dependent apical polarity. In addition, our dye-labeling approach could be utilized to sort out a certain cell population in differentiated micropatterned colonies. In summary, hPSC colonies have position-dependent differences in apical structures and properties, and actin dynamics appear to play an important role in the establishment of this position-dependent cell pola
Who reads Cell Position Within Human Pluripotent Stem Cell Colonies Determines Apical Specialization via an Actin Cytoskeleton-based Mechanism?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Kim, Youngju (author);Jang, Hwanseok (author);Seo, Kyubin (author);Kim, June Hoan (author);Lee, Boram (author);Cho, Hyo Min (author);Kim, Hyun Jung (author);Yang, Esther (author);Kim, Hyun (author);Gim, Jeong-An (author);Park, Yongdoo (author);Ryu, Jae Ryun (author);Sun, Woong (author)
- Publisher
- Elsevier BV
- Published
- 2022
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)