About this Neuroscience article

Further evidence for the anti-inflammatory activity of oleocanthal: Inhibition of MIP-1α and IL-6 in J774 macrophages and in ATDC5 chondrocytes by Morena Scotece; Rodolfo Gómez; Javier Conde; Verónica Lopez; Juan J. Gómez-Reino; Francisca Lago; Amos B. Smith; Oreste Gualillo is a Neuroscience article available to read on EtoBox.

Given the relevance of degenerative joint diseases in our society, the development of a novel pharmacologic intervention is a critically important public health goal. Recently, oleocanthal, a polyphenolic natural compound from extra virgin olive oil, has emerged as a potential therapeutic weapon for the treatment of inflammatory degenerative diseases. The goal of this study was to further evaluate the anti-inflammatory activity of oleocanthal in murine macrophages J774 and murine chondrocytes ATDC5 with a particular focus on the inhibition of gene expression of pro-inflammatory factors such as MIP-1α and IL-6. Main methods: ATDC5 murine chondrogenic cells and murine macrophages J774 were used. J774 macrophages were tested with different doses of oleocanthal and cell viability was evaluated using the MTT assay. Western blot analysis was carried on in J774 cells using anti NOS2 antibody. Nitrite accumulation was determined in culture supernatant using the Griess reaction. MIP-1α and IL-6 mRNA levels were determined using SYBR Green-based quantitative RT-PCR. MIP-1α and IL-6 protein levels were evaluated using specific ELISA assay. Several cytokines, involved in the inflammatory respo

Author

Morena Scotece; Rodolfo Gómez; Javier Conde; Verónica Lopez; Juan J. Gómez-Reino; Francisca Lago; Amos B. Smith; Oreste Gualillo

Publisher

Elsevier BV

Published

2012

Language

EN

Field

Neuroscience (Life Sciences)