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Efficient and stable gene transfer of growth factors into chondrogenic cells and primary articular chondrocytes using a VSV.G pseudotyped retroviral vector by Stephan Vogt; Peter Ueblacker; Christopher Geis; Bettina Wagner; Gabriele Wexel; Thomas Tischer; Achim Krüger; Christian Plank; Martina Anton; Vladimir Martinek; Andreas B. Imhoff; Bernd Gansbacher is a Engineering article available to read on EtoBox.
What is Efficient and stable gene transfer of growth factors into chondrogenic cells and primary articular chondrocytes using a VSV.G pseudotyped retroviral vector about?
Since efficient transfer of foreign genes into primary articular chondrocytes (CC) is difficult, a VSV.G pseudotyped retroviral vector (Bullet) was developed for marker and growth factor gene transfer. Transduction efficiency was analysed by FACS. BMP2 production was determined by specific hBMP2-ELISA. BMP2 effect on cells regarding proteoglycan production was measured by alcian blue staining and dye quantification. Alkaline phosphatase activity was determined by enzymatic reaction with p-nitrophenyl phosphate at OD 405nm and proliferation rate was analysed by MTT-assay. ATDC5 cells (98.3+/-0.6%SD) were transduced to express the reporter gene eGFP. After 52 weeks 94.7+/-0.6%SD of cells were positive. Retroviral transduction efficiency for nlslacZ exceeded 92.3+/-6.1%SD in rabbit CC and expression remained high after 15 weeks (75.7+/-14.2%SD). ATDC5 cells and CC expressed the growth factor gene hBMP2 after retroviral transduction at different time-points. BMP2 led to an increase in proteoglycan and alkaline phosphatase production. Initially, the proliferation rate detected by MTT-assay increased in both the cell types; afterwards the proliferation rate was similar to controls. The d
Who reads Efficient and stable gene transfer of growth factors into chondrogenic cells and primary articular chondrocytes using a VSV.G pseudotyped retroviral vector?
It is typically read by researchers, students, and practitioners in Engineering.
- Author
- Stephan Vogt; Peter Ueblacker; Christopher Geis; Bettina Wagner; Gabriele Wexel; Thomas Tischer; Achim Krüger; Christian Plank; Martina Anton; Vladimir Martinek; Andreas B. Imhoff; Bernd Gansbacher
- Publisher
- Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0142-9612)
- Published
- 2008
- Language
- EN
- Field
- Engineering (Physical Sciences)