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Reactivation of isolated mitotic apparatus: Metaphase versus anaphase spindles by Dr. Robert E. Palazzo; Douglas A. Lutz; Lionel I. Rebhun is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Reactivation of isolated mitotic apparatus: Metaphase versus anaphase spindles about?

Mitotic spindles isolated from sea urchin eggs can be reactivated to undergo mitotic processes in vitro. Spindles incubated in reactivation media containing sea urchin tubulin and nucleotides undergo pole-pole elongation similar to that observed in living cells during anaphase-B. The in vitro behavior of spindles isolated during metaphase and anaphase are compared. Both metaphase and anaphase spindles undergo pole-pole elongation with similar rates, but only in the presence of added tubulin. In contrast, metaphase but not anaphase spindles increase chromosome-pole distance in the presence of exogenous tubulin, suggesting that in vitro, tubulin can be incorporated at the kinetochores of metaphase but not anaphase chromosomes. The rate of spindle elongation, ultimate length achieved, and the increase in chromosome-pole distance for isolated metaphase spindles is related to the concentration of available tubulin. Pole-pole elongation and chromosome-pole elongation does not require added adenosine triphosphate (ATP). Guanosine tnphosphate (GTP) will support all activities observed. Thus, the force generation mechanism for anaphase-B in isolated sea urchin spindles is independent of add

Who reads Reactivation of isolated mitotic apparatus: Metaphase versus anaphase spindles?

Author

Dr. Robert E. Palazzo; Douglas A. Lutz; Lionel I. Rebhun

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0886-1544)

Published

1990

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)